Tue, 21 Nov 2017

Forced Degradation Study of Pyrazinamide In Bulk and Formulation by UHPLC Method

G.Sathyavani1, M.Sathish1*, A. Jerad Suresh1

1.Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College Chennai 3


ABSTRACT

The present study was undertaken to determine the forced degradation of Pyrazinamide, performed by various conditions such as acid, alkali, oxidation, thermal and photolytic. The study includes both Pyrazinamide in bulk and tablet formulation. The study based on available guidelines and main reference .Pyrazinamide has a Pyrazine nucleus. It is easily hydrolyzed by acid and alkali. The assay value of degraded products measured by intraday (30mins, 60mins, 90mins) and interday (1st, 3rd, 5th day) by UHPLC. Extensive degradation was observed in alkali hydrolysis method, and the degraded products were analysed by using UHPLC. At 90mins of intraday study using 0.1M NaOH, the degradation assay value of bulk and formulation were found to be 84.50% and 83.40% respectively. Intraday study, the degradation of bulk and formulation was observed on 1st day with the assay value of 23.62% and 25.42% respectively. However complete degradation of Pyrazinamide was observed on 3rd day and 5thday. It was determined that Pyrazinamide was found to be extremely unstable under alkali condition.

Keywords: Pyrazinamide, Kromasil C18 Column, Buffer, Acetonitrile UHPLC determination

 


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