Tue, 21 Nov 2017

Evaluation of cytotoxic and anticancer activity of prodigiosin produced by Serratia Spp.

Phatake Y.B1*, Dharmadhikari S.M2

1.Department of Microbiology, Vidya Pratishtans School of Biotechnology, Baramati, Maharashtra.

2.Department of Microbiology, Government Institute of Science, Aurangabad.


ABSTRACT

Red pigment of Bacillus prodigiosus, now known as Serratia marcescens, were first time investigated and named in 1902. The major component was first isolated in pure form in 1929 and in next 5 years investigators elucidated the main structural features of Prodigiosin and culminated in the assignment of structure. In the present study, potent pigment producing bacteria were used which were previously isolated from soil samples collected from different regions of Baramati, Maharashtra, India. The large scale production of Prodigiosin was achieved by using optimized parameters (time of incubation, temperature of incubation, pH of the medium, speed of Agitation, carbon and nitrogen source used etc.). The effect of red pigment on normal cells was assessed by toxicity studies using trypan blue assay. It shows that pigment having very less or no toxic effect on normal cells at various concentrations (5, 10, 15, 20, 25, 30, 35, 40, 45, 50 µg/ml). The effect of various concentrations (7.8, 15.6, 31.25, 62.5, 125, 250 µg/ml) of pigment on human cancerous cells (Lymphocytes) was also determined by using flow cytometry. Apoptosis was quantified by a reduction in cell forward scatter and increase in cell side scatter in flow cytometric analysis. Study revealed that the produced red pigment may be used for production of effective anticancer drug in future.

Keywords: Prodigiosin, Serratia marcescens,ccytotoxic activity, Anticancer activity, FACS.


[PDF]  Viewed: 677  Downloaded: 156
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter