Mon, 18 Dec 2017

Development and Validation of Stability-Indicating RP-HPLC Method for Determination of Fluindione

Mrinalini C. Damle*, Mahesh G. Thakare

1.AISSMS College of Pharmacy, Kennedy Road, Near RTO, Pune-411001.


ABSTRACT

Fluindione is an oral anticoagulant drug. A simple and rapid and was validated stability indicating HPLC method for Fluindione was successfully developed and was validated. This method is based on HPLC separation followed by UV detection at 285 nm. HPLC method was developed on a Symmetry thermo C18 (4.6 x 250 mm) column with a mobile phase consisting of 10mM di-sodium hydrogen phosphate buffer pH 3.5: methanol 15:85 v/v, pumped at 1.0 ml min-1 flow rate. The pH of buffer was adjusted to 3.5 with ortho phosphoric acid. The column was maintained at ambient temperature and 20μl of solutions were injected. The eluted compound was detected by using PDA detector. Fluindione was eluted at 2.4 ± 0.2 min. Stress degradation study shows that sample degraded with acid and base hydrolysis, under oxidation, thermal and photolytic stress conditions. The method was validated in accordance with requirement of ICH guidelines.

Keywords: Fluindione, Stability indicating RP-HPLC, Forced degradation, validation


[PDF]  Viewed: 284  Downloaded: 92
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter