Mon, 18 Dec 2017

Effect of d-Limonene on behavior and brain oxidative stress in cerebral-ischemia reperfusion injury of mice

Harpreet Kaur1, Nitin Bansal1*

1.Department of Pharmacology, ASBASJSM College of Pharmacy, Bela (Ropar)-140111


ABSTRACT

The present study explored the neuroprotective role of d-Limonene in the cerebral ischemia-reperfusion injury in mice. Swiss Albino mice (either sex), weighing between 20-30 g were divided in different groups (n≥6). The animals were anaesthetized using Chloral hydrate (400 mg/kg; i.p.) and cerebral blood supply was occluded for 10 min and afterwards reperfusion for 24 h. d-Limonene has been administered in 3 doses (50, 100 and 200 mg/kg; p.o.) successively to mice for 30 days before surgery. Edaravone (3 mg/kg, i.p.) was used as standard drug. The effect of surgery on memory, anxiety, muscle relaxant and locomotor was estimated by using passive avoidance paradigm, elevated zero maze, rota rod apparatus, and actophotometer. After behavioural evaluation, the animals were sacrificed and brains were isolated, homogenized and centrifuged for TBARS, GSH, catalase and brain nitrite estimations. Ishemia-reperfusion injury caused decrease in locomotor, motor-co-ordination, decreased the time spent in open arm, no. of entries and increased in latency time in elevated zero maze and memory of mice. Ischemic mice showed higher brain TBARS and nitrite levels and lower GSH and catalase levels. However, d-Limonene administration significantly attenuated (p<0.05) the behavioural and biochemical alterations produced by ischemia-reperfusion injury. d-Limonene treated mice showed a significant (p<0.05) decrease in TBARS level and brain nitrite levels. Therefore, d-Limonene may prove to be a beneficial agent in the prevention of stroke.

Keywords: d-Limonene, ischemia-reperfusion injury, bilateral carotid artery occlusion, oxidative stress.


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