Thu, 14 Dec 2017

Isolation of Fetal Cells from Maternal Circulation Using Magnetic Cell Separation

Radhakrishna Nagumantri1, Animisha Mokkapati1, Radha Tatapudi2, Madhuri Chintala2, Satyanarayana Rentala1, * , Aruna Lakshmi Komarraju1

1. Department of Biotechnology, Institute of Technology, GITAM University, Visakhapatnam 530 045, Andhra Pradesh, INDIA

2. Department of Gynecology and Obstetrics, Andhra Medical College, Visakhapatnam 530 002, Andhra Pradesh, INDIA


ABSTRACT

Prenatal diagnosis employs a variety of techniques to determine the health of fetus. Without knowledge gained by prenatal diagnosis, there could be an untoward outcome for the fetus or the mother or both. There are a variety of non-invasive and invasive techniques available for prenatal diagnosis. Each of them can be applied only during specific time periods during the pregnancy for greatest utility. All current methods of fetal karyotyping are invasive and carry a definite, albeit small, procedure-related risk. Because of this and testing costs, only women older than 35 years who have a greater risk for fetal aneuploidy are currently offered prenatal testing. The isolation and analysis of fetal cells from maternal blood would allow non-invasive prenatal genetic screening and diagnosis. One method that is currently being explored involves culturing fetal cells. Developing conditions which allow the number of fetal-derived cells to expand in culture and the number of maternally derived cells to be suppressed in culture may lead to a new selection process for obtaining fetal cells. In this paper the isolation of fetal cells using magnetic cell sorter methods was discussed. It was found that 5000 cells were present in 28 million maternal cells.

Keywords: Prenatal diagnosis, fetal cells, markers for fetal cells , isolation of fetal cells and culturing of fetal cells


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