DOI: 10.21276/ajptr
Mon, 27 May 2019

In-vitro cytoxicity of M.Maderspantana (Linn.) Cogn. Fruit Methanol Extract against MCF 7 Human Breast Cancer Cell Line and Quantification of Beta Carotenoid in HPLC

A.Suganthi1*, R.Mary Josephine 1

1.Department of Botany, Nirmala College for Women Coimbatore  Tamil Nadu, India


The objective of the study was to determine the anticancer efficacy of M. maderaspatana fruit extract against Michigan Cancer Foundation-7 (MCF-7). Different concentration (10, 20, 30, 40, and 50 μg/ml) of methanol extract of M. maderaspatana fruit were used to assess the in vitro cytotoxic effect using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Identification and quantification of beta carotenoid in HPLC. The various concentrations of crude methanol extract (10, 20, 30, 40, and 50 μg/ml concentration) of M. maderaspatana fruit were performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against Michigan Cancer Foundation-7 (MCF-7)   with an inhibit cell growth by 50% (IC50) of 32 ± 1.0 μg/ml. The βeta- carotene content through spectrophotometry and high performance liquid chromatography HPLC analysis showed that 88ppm of betacarotene present in M.maderspantana fruits.  The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the M.maderspantana extract indicates that has anticancer activity against Michigan Cancer Foundation-7 (MCF-7)   cancer cell lines. The present study concluded that the methanol extract of M.maderspantana possess potent cytotoxic activity.

Keywords: MCF – 7 Breast cancer cell line, M.maderspantana, Fruit, Beta carotenoid

[PDF]   Viewed: 532   Downloaded: 64
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter