Sat, 18 Nov 2017

Analytical RP- HPLC Method Development and Validation for Simultaneous Estimation of Azilsartan Medoxomil and Chlorthalidone In Pharmaceutical Dosage Form

K.Lavanya*1, V.Srinivasa Rao1, P.Sunitha2, K.Pavani Sai Rama2

1. Department of pharmaceutical Analysis, Lydia College of Pharmacy, Ravulapalem-533238.

2. Department of pharmaceutical Analysis, Koringa College of Pharmacy, Korangi- 533461


ABSTRACT

A reverse phase HPLC method is developed for the determination of Azilsartan medoximil and chlorthalidone in pharmaceutical dosage forms. Chromatography was carried out on a C18 column 4.6 x 100mm, 5µm, Make: BDS using a mixture of potassium Di hydrogen ortho phosphate buffer and acetonitrile (35:65 v/v) as the mobile phase at a flow rate of 1.0 ml/min. Detection was carried out at 273 nm . The retention time of Azilsartan Medoxomil and Chlorthalidone was 2.59±0.1 mins and 3.85±0.5 min respectively. The linearity was observed In range of 2.5-15 µg/ml and 10-60 µg/ml with a correlation coefficient of Azilsartan medoximil and chlorthalidone were 0.996 and 0.999.the proposed method was validated for its linearity, accuracy, precision and robustness. The proposed method was found to be simple, rapid, accurate and precise. It was found to be economical and suitable for simultaneous determination of Azilsartan Medoxomil and Chlorthalidone in pharmaceutical dosage form.

Keywords: HPLC, Azilsartan medoximil and Chlorthalidone, Estimation, Tablets.


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