DOI: 10.21276/ajptr
Sat, 25 May 2019

High Performance Liquid Chromatography and Its Validation – Review

Kaushik Vilas Kulkarni*, Daridevanand Baburao Patil, Akshay Sudhakar Dhupad

D.S.T.S Mandal’s college of pharmacy, Jule Solapur, Solapur.


HPLC is the dominant separation technique in modern pharmaceutical and biomedical analysis because it results in highly efficient separations and in most cases provides high detection sensitivity. Most of the drugs in multi component dosage forms can be analyzed by HPLC method because of the several advantages like rapidity, specificity, accuracy, precision and ease of automation in this method. HPLC methods development and validation play important roles in new discovery, development, manufacture of pharmaceutical drugs and various other studies related to humans and animals. It is the most versatile, safest, dependable and fastest chromatographic technique for the quality control of drug components. This article was prepared with an aim to review different aspects of HPLC, such as principle, types, instrumentation, validation of HPLC and application. 

Keywords: High performance liquid chromatography, instrumentation, validation of HPLC, applications.

[PDF]   Viewed: 422   Downloaded: 86 DOI No: 10.21276/ajptr.2018.08.02.07
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