DOI: 10.21276/ajptr
Fri, 18 Jan 2019

Development and Validation of Stability Indicating RP-UPLC Method for Quantitative Estimation of Lamivudine in Tablet Dosage Form

T. Benjamin1*, D. Ramachandran2

1.Department of Chemistry, Noble College, Krishna University, Machilipatnam. Andhra Pradesh, India

2.Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur. Andhra Pradesh, India


ABSTRACT

A new, simple, rapid, selective, precise and accurate isocratic reverse phase ultra performance liquid Chromatography assay method has been developed for estimation of Lamivudine in tablet formulations. The separation was achieved by using column Acquity UPLC BEH Phenyl (100×2.1mm, 1.7µm), in mobile phase consisted of pH 3.8 ammonium acetate buffer and methanol. The flow rate was 0.5mL.min-1 and the separated Lamivudine was detected using UV detector at the wavelength of 277 nm. The retention time of Lamivudine was noted to be 2.50 min respectively, indicative of rather shorter analysis time. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.

Keywords: Liquid chromatography; Lamivudine, Validation and Reproducible.

 


[PDF]   Viewed: 19   Downloaded: 20 DOI No: 10.21276/ajptr.2018.08.06.07
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